raf 1 antibody Search Results


raf1  (Bioss)
90
Bioss raf1
Raf1, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti craf antibody  (R&D Systems)
91
R&D Systems anti craf antibody
Anti Craf Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti raf 1  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti raf 1
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
Anti Raf 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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raf1  (Proteintech)
95
Proteintech raf1
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
Raf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a301 519a  (Bethyl)
93
Bethyl a301 519a
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
A301 519a, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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raf antibody  (Proteintech)
93
Proteintech raf antibody
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
Raf Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p raf 1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology p raf 1
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
P Raf 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mek1 2 proteintech 11049 1 ap  (Proteintech)
93
Proteintech mek1 2 proteintech 11049 1 ap
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
Mek1 2 Proteintech 11049 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phosphoser621  (OriGene)
90
OriGene anti phosphoser621
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
Anti Phosphoser621, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal anti phosphorylated p c raf  (Bioss)
91
Bioss rabbit monoclonal anti phosphorylated p c raf
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
Rabbit Monoclonal Anti Phosphorylated P C Raf, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal anti phosphorylated p c raf - by Bioz Stars, 2026-03
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rabbit antihuman polyclonal raf 1 antibody  (Sino Biological)
93
Sino Biological rabbit antihuman polyclonal raf 1 antibody
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
Rabbit Antihuman Polyclonal Raf 1 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated human ampkα2 β2 γ1  (Carna Inc)
96
Carna Inc phosphorylated human ampkα2 β2 γ1
Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an <t>anti-Raf-1</t> antibody.
Phosphorylated Human Ampkα2 β2 γ1, supplied by Carna Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an anti-Raf-1 antibody.

Journal:

Article Title: Growth Factor-Independent Proliferation of Erythroid Cells Infected with Friend Spleen Focus-Forming Virus Is Protein Kinase C Dependent but Does Not Require Ras-GTP

doi:

Figure Lengend Snippet: Expression of dominant-negative Ras in uninfected and SFFV-infected HCD-57 cells transfected with H-rasN17. Cell lysates were prepared for Western blot analysis from uninfected and SFFVP-infected HCD-57 cells cotransfected with a vector expressing the dominant-negative H-rasN17 mutant (lanes 2, 4, and 6) or a control vector (lanes 1, 3, and 5) plus the pEGFP-N2 N-terminal fusion vector expressing GFP and the neomycin resistance gene. Cells expressing GFP were purified by FACS and evaluated for H-rasN17 expression 2 days after transfection (lanes 1 to 4) or 3 months after G418 selection (lanes 5 and 6) using an anti-Ras antibody that detects both endogenous and mutant forms of the Ras protein. Protein loading was determined by immunoblotting with an anti-Raf-1 antibody.

Article Snippet: For Raf-1 and MAPK immune complex kinase assays, protein was purified from 1 mg of lysate using anti-Raf-1 or Erk-2 polyclonal (C14) antibodies from Santa Cruz Biotechnology.

Techniques: Expressing, Dominant Negative Mutation, Infection, Transfection, Western Blot, Plasmid Preparation, Mutagenesis, Purification, Selection

PKC is required for activation of MAPK but not Raf-1 or MEK in uninfected and SFFV-infected HCD-57 cells. Uninfected and SFFV-infected HCD-57 cells were treated with the PKC inhibitor staurosporine (Stauro) (40 nM) or bisindolylmaleimide (Bis) (20 μM) or the PKA inhibitor HA1004 (40 μM). Uninfected HCD-57 cells were then stimulated with Epo for 15 min, while SFFV-infected HCD-57 cells were left unstimulated. Cell lysates were assayed in immune complex assays for MAP kinase activity (A), Raf-1 kinase activity (B), and MEK kinase activity (C). Protein loading was determined by immunoblotting with the appropriate antibody. MEK−, GST-kinase-inactive MEK.

Journal:

Article Title: Growth Factor-Independent Proliferation of Erythroid Cells Infected with Friend Spleen Focus-Forming Virus Is Protein Kinase C Dependent but Does Not Require Ras-GTP

doi:

Figure Lengend Snippet: PKC is required for activation of MAPK but not Raf-1 or MEK in uninfected and SFFV-infected HCD-57 cells. Uninfected and SFFV-infected HCD-57 cells were treated with the PKC inhibitor staurosporine (Stauro) (40 nM) or bisindolylmaleimide (Bis) (20 μM) or the PKA inhibitor HA1004 (40 μM). Uninfected HCD-57 cells were then stimulated with Epo for 15 min, while SFFV-infected HCD-57 cells were left unstimulated. Cell lysates were assayed in immune complex assays for MAP kinase activity (A), Raf-1 kinase activity (B), and MEK kinase activity (C). Protein loading was determined by immunoblotting with the appropriate antibody. MEK−, GST-kinase-inactive MEK.

Article Snippet: For Raf-1 and MAPK immune complex kinase assays, protein was purified from 1 mg of lysate using anti-Raf-1 or Erk-2 polyclonal (C14) antibodies from Santa Cruz Biotechnology.

Techniques: Activation Assay, Infection, Activity Assay, Western Blot